8.5 Reproducibility

The computational analysis of RNA-seq count tables demonstrated in this chapter require the following R library dependencies (only listing libraries outside of base R).

• DESeq2: Differential expression analysis
• RUVseq and EDASeq: Removing unwanted variation and diagnostics
• gProfileR: Functional term enrichment analysis
• Visualization:
  • ggplot2
  • ggfortify
  • pheatmap
  • corrplot
  • knitr

DESeq2, RUVSeq, and EDASeq can be installed from the Bioconductor repository via:

if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")
BiocManager::install(c('DESeq2', 'RUVSeq', 'EDASeq'))

The remaining package can be installed from CRAN via:

install.packages(c('gProfileR', 'ggplot2', 'ggfortify', 'pheatmap', 'corrplot', 'knitr'))